The genus Weissella: taxonomy, ecology and biotechnological potential. Please enable it to take advantage of the complete set of features! The qM-H values given by equation 13, these vary from 0.9993 to 0.9841 for the range between 8.8 to 200kDa in a temperature range of 20 to 40C. Gekko, K. (1971). Keywords: intrinsic viscosity, dextrans, Mark-Houwink parameters, hydrodynamic, temperature, Journal of Polymer and Biopolymer Physics Chemistry, 2013 1 (1),
Epub 2017 Jun 1.
Dextrans in Aqueous Solution. Experimental Review on Intrinsic Department of Biomedical-Chemical Engineering, The Catholic University of Korea, 43 Jibong-ro Wonmi-gu, Bucheon-si, Gyeonggi-do, 14662, Republic of Korea, LG electronics, 19 Yangjae-daero 11-gil, Seocho-gu, Seoul, 06772, Republic of Korea, You can also search for this author in ; Bos, G.W.
Influence of molecular weight on dextran Use of the single point method is correct because it is based in the Huggins method (HM); owing the HM requiring many viscosity and density measurements (at least four) for each Mw, but Solomon Ciuta method with a single measure is sufficient. 24h after microporation, the pore sizes reduced from 64.8817.20m (0h) to 48.854.27m (24h), and the pore depths reduced from 83.9120.56m (0h) to 63.9527.61m (24h). For a limited temperature range, the viscosity of a polymer solution varies generally following a relation similar to that of usual liquids (rrhenius plot), where the polymer concentration is not very high and that temperature remains far enough from glass transition [38], and for system PEAorganic solvents see reference [63]. The Transdermal diffusion cell system was protected from light. Nordmeier, Eckliard (1993). Monosaccharide analysis revealed that the polysaccharide comprised only glucose units. The intrinsic viscosity classical theory of random coils at conditions []0 predicts the quotient a0 = d ln[]0/d ln M to decrease from unity to 0.5 as the chain length increases. 2012;27(3):20212. Peptide and protein transdermal drug delivery. The cumulative permeation of FITC-dextran 10 K was 4.82 0.32 M, in the human skin and 5.92 0.46 M in the pig skin. Chen Rong Huei; Wei Yu Chen; Shang Ta Wang; Chu Hsi Hsu; Min Lang Tsai (2009). Harding, Stephen E. (1997). J. Braz. It is worth noting that the size of macromolecules is much bigger than that of solvent (water) molecules [50]. Carbohydrate Polymers 88 373-381. (2007). Recent developments in polysaccharide-based materials used as adsorbents in wastewater treatment. The enhanced permeability through micropores was confirmed by Franz diffusion cell.
Hydrodynamic Radius The slope is a value and intercept is equal to the ln kMH value [47]. The numerical value of a indicates that dextrans acquire the shape of a rigid sphere in aqueous solution; and kM-H demonstrates that under water the value decreases with temperature [71, 72]. Hi Khanh, Alan is taking a little time off of Research Gate at the moment, but here is some information in reply to your question. Bahary, W. S.; Hogan, M. P.; Jilani, M.; Aronson, M. P. (1995). Kloster, C.; Bica, C.; Lartigue, C.; Rochas, C.; Samios, D.; Geissler E. (1998). These studies were carried out at the Hisko (Gunpo, Korea). These structural differences can significantly affect FITC-dextran delivery due to even after microporation [30]. The dextran has the advantage in terms of low cytotoxicity, excellent biocompatibility, and water-solubility. 2005;22(4):5505. This site needs JavaScript to work properly. Unperturbed dimensions and theta temperature of dextran in aqueous solutions. Keywords: Pribush, A.; Zilberman-Kravits, D.; Meyerstein, N. (2007). Tirtaatmadja, V.; Dunstan, D. E.; Boger, D. V. (2001). Food Funct. Carbohydrate Polymers, 79, 380-390. Molecular weight determination of polysaccharides. (more or less, with a hydrodynamic radius of less than 100 nm) to cross from the vasculature into the interstitium. The intrinsic viscosity calculation requires several concentrations, and because of this inconvenient will not use the Huggins method in this paper. The permeability of fluorescein isothiocyanate-dextrans (FITC-dextrans) with different molecular weights through the pig and human cadaver skins were measured using Franz diffusion cell.
Modelling the endothelial blood-CNS barriers: a method for the (1999). Surface morphology study of dextran by scanning electron microscopy showed the porous web like structure. Molecular weight, molecular weight distribution and molecular size of a native dextran. The study of biopolymers as dextran in aqueous solution, is effectively determined by intrinsic viscosity [] measurements at different temperatures. The receptor phase was collected immediately (time 0) and 2, 4, 8, 12, and 24h. This receptor phase solution was transferred to a 96-well plate and the skin-permeated FITC-dextran was measured using a microplate reader (Molecular Devices, Co. Ltd., Sunnyvale, CA, USA) at 405nm. Many works are aimed at determining molecular weights [1, 7, 26], hydrodynamic radius, among other parameters for characterizing the physics and chemistry properties of dextran in solution. Each microporated sample was incubated in the skin culture medium. Trop J Pharm Res. 1994, 95-0355. For a rigid or rod like polymer molecule that is greatly extended in solution, the Mark-Houwink a constant approaches to a value of 2.0 [48].The hydrodynamic radius (RH), for a sphere ((a/b) = 2.5) is given by the Einstein relation [49]. 2014 Sep;5(9):2324-30. doi: 10.1039/c4fo00319e. Pharmaceut Sci Tech. However, the currently available transdermal systems are limited by low drug permeability across the skin due to the lipophilic barrier function of the stratum corneum (SC), the outermost skin layer of the skin [3,4,5]; the methods favor low molecular mass, and lipophilic molecules, making it difficult to exploit the transdermal route for large and water-soluble molecules such as proteins and peptides. Carbohydrate Polymers, 38, 267-271. Ann Surg Oncol. We observe that this maximum radius is below Drug Deliv. These results indicate that healing and regeneration occurred in the human cadaver skin after the microporation. The flattening of the curves at higher molecular weight is in agreement with an increased branching density. Webwhittier union high school district superintendent. The authors declare that they have no competing interests. Intrinsic viscosity-molecular weight relationship for chitosan. Viscosity of in water solution polysaccharides depends on intrinsic characteristics of the biopolymer (such as molecular weight, volume, size, shape, surface charge, deformation facility, esterification degree, and galacturonic content) and on ambient factors (such as pH, temperature, ionic strength, solvent, etc.). TEWL measurements were taken to assess the integrity of the skin barrier and the extent of SC disruption. Determination of intrinsic viscosities of macromolecules and nanoparticles. Transdermal drug delivery system for non-steroidal anti inflammatory drugs: a review. Middelkoop E, Bogaerdt AJ, Lamme EN, Hoekstra MJ, Brandsma K, Ulrich MMW. Biological skin substitutes for wound cover and closure. DOI: 10.12691/jpbpc-1-1-3, Received September 18, 2013; Revised October 23, 2013; Accepted November 22, 2013.
Dilute solution properties and degree of chain branching 2003;89(2):31120. WebIn this work, hydrodynamic properties are investigated in different dextran aqueous solutions with different molecular weights (8.8-2000kDa), in a temperature range from 20C to 50C. These values indicate for dextrans in dilute aqueous solution, both methods give very similar results of the intrinsic viscosity and the respective Mw.
dextran Its Invariancy with Respect to the Cross-Sectional Dimensions of Polymer Chains. Journal of Applied Polymer Science, 13, 2031-2032. A lot of research has been devoted to dextrans modification in order to describe special characteristics relevant to specific applications such as surfactants [16], as visible marker [17], polysaccharides-based nanoparticles, covalent crosslinking [18], ionic crosslinking, polyelectrolyte complex, and the self-assembly of hydrophobically modified polysaccharides, and hydrophilic modified polysaccharides [19]. The .gov means its official. Nat Biotechnol. California Privacy Statement, Article Advanced Drug Delivery Reviews, 60, 1650-1662. A simple and rapid method for evaluation of MarkHouwinkSaturada constants of linear random coil polysaccharides using molecular weight and intrinsic viscosity determined by high performance size exclusin chromatography: application to guar galactomannan.
Hydrodynamic Lucifer yellow (LY) and FITC-labelled dextran stock solutions were prepared in Ringers-HEPES buffer (150 mM NaCl, 3.4 mM CaCl 2, 1.2 mM MgCl 2, 5.2 mM KCl, 0.5 mM NaHCO 3, 2.8 mM glucose, and 10 mM HEPES) and frozen at 20C.
Nanoparticles in magnetic resonance imaging: from simple to dual Dextran is soluble in water, methyl sulphoxide, formamide, ethylene glycol, glycerol, 4-methylmorpholine-4-oxide, and hexamethyl phosphamide [4]. FITC-dextran molecular weights; 4, 10, and 20kDa molecular weight were purchased from Sigma (St. Louis, MO, USA). For data requests, please contact the authors. 2019;9(3-S):9726. Mark-Houwink values confirm that for these conditions dextrans behave as rigid spheres with a tendency at compaction as temperature increases. Beer, M.U. Chapter 2.1.4 Suspended level viscometers for transparent liquid, Ubbelohde Viscometer page 28-31. Pavlov, G.M. (2013). 8. The lower molecular weight of FITC-dextran permeated more on both human and pig skin. Physicochemical studies of oligodextran. The column was eluted with bidestilled water a flow rate of 0.8 ml/min, injection 5L, back pressure 200psi. Masuelli, Martin Alberto (2013). Journal of Polymer Science, 17, 527-546.
What is hydrodynamic radius ; Patel, T.R. WebFluorescein isothiocyanatedextran average mol wt 60,000-76,000; CAS Number: 60842-46-8; Synonyms: FITCDextran; find Sigma-Aldrich-FD70S MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich pp13-21. WebDextran 40 Pharmaceutical Quality. The explanation for the anomalous values of a and kM-H is because the mixture solute-solvent is highly compatible due a great compaction of biopolymer, from undisturbed state in the absence of interactions, where free energy of mixing less than zero, and emphasize the nature non-linear or hyperbranched of dextran for Mw high to 200kDa. Learn More. WebDextran produced from Weissella cibaria JAG8 was purified and characterized. Weissella: An Emerging Bacterium with Promising Health Benefits. Effect of temperature on the intrinsic viscosity and conformation of chitosan in dilute HCl solution. 2012;101(8):28619. ; De Jong, S.J. WebThe invention relates to novel fluorescent dyes with multiple negatively charged groups in their ionized form which are 9-aminoacridines or 1-aminopyrene shaving of one of the fol Stenekes, R.J.H. After 24h, the cumulative permeation of FITC-dextran M.W. In order to confirm the molecular weight the gel permeation chromatography (GPC) for standard dextrans is used in a range of molecular weights from 8.8 to 2000kDa and these correspond to the following equation. Learn More. Chemical penetration enhancers for transdermal drug delivery systems. Gold MH, Biron JA. 2012;3(1):208. 1984;17(6):11703. The Mark-Houwink a constant is close to 0.5 in poor solvents. sharing sensitive information, make sure youre on a federal Carbohydrate Polymers 95 360-365. Senti, F. R.; Hellman, N. N.; Ludwig, G.; Babcock, E.; Tobin, R.; Class, C. A., (1955). Naik A, Kalia YN, Guy RH. Curvale, R.; Masuelli, M.; Perez Padilla, A. Catiker, E.; Guner, A. Walkenhorst, S.; Olivier, R. (2002). The range of temperatures used was 25-50 (0.1) C and it was regulated by thermostatic bath Haake C. The viscosity measurements required Hagenbach-Couette correction time (tg 37.31s). FITC-dextran solution (600L) was added to the donor chamber. Carbohydrate Polymers 99, 199-207. This low polydispersity realizes that the Mark-Houwink equation is forced at a given Mw regardless of the hyperbranched nature of the dextran molecule. 10% neutral buffered formalin was purchased from Hisko (Gunpo, Korea). The parameters of Mark-Houwink for polymers can vary with the polymer solution and temperature. Synthesis and characterization of blue dextrans.
Hydrodynamic Food Sci Biotechnol. The hydrodynamic 57 radius of the dextranase is 7 Transdermal delivery is of great importance for the effective delivery of bioactive or therapeutic agents into a body. J. Braz. Hindered diffusion of dextran and ficoll in microporous membranes. Santoro MM, Gaudino G. Cellular and molecular facets of keratinocyte reepithelization during. Kloster, C.; Bica C.; Rochas C.; Samios, D.; Geissler, E. (2000). Although, intrinsic viscosity is a molecular parameter that can be interpreted in terms of molecular conformation, it does not offer as high resolution on molecular structure as other methods such as Light Scattering, Circular Dichroism, Sedimentation Velocity, Sedimentation Equilibrium, Size Exclusion Chromatography Coupled to Multi-Angle Laser Light Scattering (SEC-MALLS), High-performance Size Exclusion Chromatography (HPSEC), Gel Permeation Chromatography (GPC), NMR, X-rays, etc. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was Carbohydrate Polymers, 36, 319-326. a Microscopy images of micropore and b quantified data using ImageJ after 0 (immediately), 4, 8, and 24h according to the number of times microproration was done (n=150, *: p<0.05). Journal of Polymer Science Part B: Polymer Physics, 38, 2591-2598. Fusco V, Quero GM, Cho GS, Kabisch J, Meske D, Neve H, Bockelmann W, Franz CM. The Intrinsic Viscosity of Biological Macromolecules. Transdermal delivery of FITC-Dextrans with different molecular weights using radiofrequency microporation, https://doi.org/10.1186/s40824-020-00201-7, http://creativecommons.org/licenses/by/4.0/, http://creativecommons.org/publicdomain/zero/1.0/. Pharm Res. All authors read and approved the final manuscript. 2015;32(5):170413. This result could reflect the presence of some aggregated species within the lysozyme sample solution. Typically, the hydrodynamic diameter of the micelles is about 100 nm and the critical micelle concentration is about 10 mg/L. Front Microbiol. Eng. 2009;8(2):1739. For simplicity, macromolecules are treated as rigid molecules for a hydrodynamic study. Dextran molecular size and degree of branching as a function of sucrose concentration, pH, and temperature of reaction of. ; Hogan, M.P. WebR H Hydrodynamic radius [m] The basic DLS setup. WebDextran displays excellent biocompatibility and have been used clinically for over 50 years. Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. The skin poration was performed using the microporation device (LG electronics, Korea). Dextran (DX) is one type of EPS synthesized by LAB in the presence of sucrose (R g), radius of hydrodynamic (R h), polymer conformation parameter ( = R g /R h), intrinsic viscosity ([]), Mark-Houwink coefficient () and branching degree of DXs of RPYs prepared under different conditions. 2017 Dec 12;27(1):73-78. doi: 10.1007/s10068-017-0274-0.
Ficoll and dextran vs. globular proteins as probes for testing The effect of branching is best recognized from the molecular weight dependencies of []. Intrinsic viscosity of bovine serum albumin conformers. official website and that any information you provide is encrypted Kuge Takashi; Kimie Kobayashi; Shinichi Kitamura; Hiroshi Tanahashi. WebHydrodynamic radius = variable size of an analyte with specific MW based on dynamical properties. Determination of branching in biopolymers by size exclusion chromatography with light scattering (Malls), viscosity, and refractive index detection. Rheology of dextran solutions. In addition, we evaluated the regeneration of SC after microporation. The hydrodynamic radius of 2-NBDG glucose was too small to measure. The analysis of errors using the Solomon-Ciuta method is compared with HM [59]. It has been found that, when applied to water treatment, it can effectively act on refractory organic matter in wastewater through thermal decomposition and water molecular action to produce free Article WebLabeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the J Pharm Sci. Normally, the skin loses very little water loss whereas damaged skin loses more [22]. Provided by the Springer Nature SharedIt content-sharing initiative. It has been found that, when applied to water treatment, it can effectively act on refractory organic matter in wastewater through thermal decomposition and water molecular action to produce free (2007). J Appl Toxicol. The most widely applied coatings for FDA-approved SPIONs are dextran and carboxydextran. Chem. Part of European Biophysical Journal, 36, 85-94. FITC-dextran solution was prepared at 1mM in PBS. Mitragotri S. Mechanical disruption of skin barrier for vaccine delivery. A novel high dextran yielding Weissella cibaria JAG8 for cereal food application. Optical properties of dextran in solution and in films. Micropores are produced by the arrangement of microelectrodes on the skin at precise dimensions, then RF energy generated by alternating currents induces ionic vibrations between electrodes with positive or negative charges. European Polymer Journal, 37, 619-622. Journal of Pharmaceutical and Biomedical Analysis, 41, 1260-1267. Boudry I, Blanck O, Cruz C, Blanck M, Vallet V, Bazire A, Capt A, Josse D, Lallement G. Percutaneous penetration and absorption of parathion using human and pig skin models in vitro and human skin grafted onto nude mouse skin model in vivo. FITC-derivatives have been used for a variety of biological applications for more than 40 yeras. The Hydrodynamic Radii of Macromolecules and Their Effect on Red Blood Cell Aggregation. WebDiffusivity of 2000 kDa Dextran was significantly lower for both hydrogel formulations, but was insensitive to collagen concentration, likely due to a larger hydrodynamic diameter ( dh ~ 70 nm) on order of the collagen mesh size. Aqueous solutions of amphiphilic polysaccharides: Concentration and temperature effect on viscosity. (1954). High Manufacturing Standards of Molecular Probes Dextrans. In fact, short chains do contain minor branching points. Carbohydrate Research, 160, 205-214. WebHydrodyamic Radius, R H: In addition to the analytic size of a linear (C=1) chain, the end to end distance, R eted or R 0, and the structural size, the radius or gyration, R g, dynamic measurements yield a size called the hydrodynamic radius, R H. Consider a rod particle of length L=10R where R is the radius.